5EPC
Crystal structure of wild-type human phosphoglucomutase 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2015-06-06 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00001 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 172.590, 172.590, 99.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.051 - 1.850 |
R-factor | 0.171 |
Rwork | 0.170 |
R-free | 0.19750 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3pmg |
RMSD bond length | 0.007 |
RMSD bond angle | 0.998 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.051 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.163 | 3.510 |
Number of reflections | 150855 | |
<I/σ(I)> | 15.1 | 1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 32.5 | 27.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Lithium or ammonium sulfate (1.35 - 1.85 M) with 0.1 M Tris, pH 8.0 |