5EP6
The crystal structure of NAP1 in complex with TBK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 298.15 |
Detector technology | CCD |
Collection date | 2004-06-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.360, 50.113, 84.416 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.092 - 1.451 |
R-factor | 0.1883 |
Rwork | 0.187 |
R-free | 0.21740 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.994 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.450 |
Number of reflections | 31428 |
<I/σ(I)> | 27.5 |
Completeness [%] | 98.8 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291.15 | 1% w/v Tryptone, 0.05 M HEPES sodium (pH 7.0), 12% w/v PEG 3350 |