5EP6

The crystal structure of NAP1 in complex with TBK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	298.15
Detector technology	CCD
Collection date	2004-06-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	41.360, 50.113, 84.416
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.092 - 1.451
R-factor	0.1883
Rwork	0.187
R-free	0.21740
RMSD bond length	0.006
RMSD bond angle	0.994
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.450
Number of reflections	31428
<I/σ(I)>	27.5
Completeness [%]	98.8
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291.15	1% w/v Tryptone, 0.05 M HEPES sodium (pH 7.0), 12% w/v PEG 3350