5EP5
The crystal structure of the hypothetical protein SAV0944 mutant (Glu47Ala) from Staphylococcus aureus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.220, 91.570, 65.740 |
| Unit cell angles | 90.00, 99.52, 90.00 |
Refinement procedure
| Resolution | 46.000 - 2.900 |
| R-factor | 0.21991 |
| Rwork | 0.217 |
| R-free | 0.26284 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.472 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 10339 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 89.7 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 1.5M Lithium sulphate, 0.1M HEPES Sodium pH7.5 |
