5EOU

Pseudomonas aeruginosa PilM:PilN1-12 bound to ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750
Spacegroup name	P 3 2 1
Unit cell lengths	131.790, 131.790, 78.993
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.497 - 2.400
R-factor	0.2137
Rwork	0.212
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eoy
RMSD bond length	0.003
RMSD bond angle	0.639
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1639)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	37322
<I/σ(I)>	21.2
Completeness [%]	100.0
Redundancy	19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	ammonium sulphate, tris