5EOP
Crystal structure of human Angiogenin at 1.35 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0403 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.664, 37.559, 33.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.830 - 1.350 |
| R-factor | 0.14968 |
| Rwork | 0.147 |
| R-free | 0.19155 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b1i |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.892 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.830 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.057 | 0.481 |
| Number of reflections | 23977 | |
| <I/σ(I)> | 12.7 | 3 |
| Completeness [%] | 98.7 | 99 |
| Redundancy | 4.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 289 | Malic acid/NaOH pH 7.0 PEG 3350 |
