5EOJ

Crystal structure of an antiparallel hexamer coiled-coil - ACC-Hex-PheI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977
Spacegroup name	P 43 2 2
Unit cell lengths	40.850, 40.850, 118.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.608 - 2.115
R-factor	0.2456
Rwork	0.244
R-free	0.27530
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.638
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.610	2.191
High resolution limit [Å]	2.115	2.115
Rmerge	0.026	0.451
Number of reflections	6246
<I/σ(I)>	13.42	1.12
Completeness [%]	98.0	99
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	5 combined crystals from a range of crystal growing conditions: 0. 1 M Na formate ph 6.75 - 7.3 and PEG 3350 25-28%