5EOF

Crystal structure of OPTN NTD and TBK1 CTD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	298.15
Detector technology	CCD
Collection date	2015-06-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	36.321, 54.775, 153.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.344 - 2.050
R-factor	0.2094
Rwork	0.207
R-free	0.24540
RMSD bond length	0.007
RMSD bond angle	0.960
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.050
Number of reflections	19991
<I/σ(I)>	14.85
Completeness [%]	99.9
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291.15	0.1M MES monohydrate pH 6.0, 14% w/v polyethylene glycol 4000