5EOD
Human Plasma Coagulation FXI with peptide LP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2012-10-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 81.065, 81.065, 253.084 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.400 - 3.100 |
R-factor | 0.19353 |
Rwork | 0.189 |
R-free | 0.27393 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.826 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.400 | 3.130 |
High resolution limit [Å] | 2.970 | 2.970 |
Rmerge | 0.149 | 0.714 |
Number of reflections | 18287 | |
<I/σ(I)> | 12.5 | 3.1 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 9.8 | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1M HEPES pH7.5, 20% PEG1500, |