5EOC
Crystal structure of Fab C2 in complex with a Cyclic variant of Hepatitis C Virus E2 epitope I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-12 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 54.972, 56.191, 77.153 |
Unit cell angles | 90.35, 90.18, 94.98 |
Refinement procedure
Resolution | 15.000 - 1.980 |
Rwork | 0.234 |
R-free | 0.26565 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2Y5T AND 2Y6S |
RMSD bond length | 0.014 |
RMSD bond angle | 1.568 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.050 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.077 | |
Number of reflections | 59746 | |
<I/σ(I)> | 15.9 | |
Completeness [%] | 93.0 | 81.7 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium sulphate and 25% (w/v) PEG 3,350 |