5EO4

Structural and biochemical characterization of the hypothetical protein SAV2348 from Staphylococcus aureus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	I 1 2 1
Unit cell lengths	63.070, 62.660, 149.357
Unit cell angles	90.00, 92.47, 90.00

Refinement procedure

Resolution	44.430 - 2.000
R-factor	0.1823
Rwork	0.180
R-free	0.22360
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.000
High resolution limit [Å]	2.000
Number of reflections	39242
<I/σ(I)>	11.91
Completeness [%]	99.3
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	296	0.2M ammonium tartrate dibasic pH6.2, 26% PEG3350