5ENY
Ketosynthase from module 6 connected to acyl carrier protein from module 5 (unobservable) of the bacillaene synthase from Bacillus subtilis 168
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 63.106, 112.732, 211.441 |
Unit cell angles | 104.96, 90.07, 106.32 |
Refinement procedure
Resolution | 39.770 - 4.000 |
R-factor | 0.33451 |
Rwork | 0.333 |
R-free | 0.35400 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.852 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.770 |
High resolution limit [Å] | 4.000 |
Number of reflections | 23712 |
<I/σ(I)> | 6.1 |
Completeness [%] | 98.7 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 25 mg/ml PksACP5+KS6, 150 mM NaCl, 10% glycerol, 10 mM HEPES, pH 7.5) with 1 uL crystallization buffer (30% PEG 4000 (v/v), 0.2 M sodium acetate, 0.1 M Tris, pH 8.5 |