5ENO
MBX2319 bound structure of bacterial efflux pump.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.758, 145.147, 174.243 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.2016 |
| Rwork | 0.199 |
| R-free | 0.24706 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5en5 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.725 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.307 | 1.630 |
| Number of reflections | 140556 | |
| <I/σ(I)> | 5.27 | |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 13.6 | 12.2 |
| CC(1/2) | 0.984 | 0.473 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES pH6.5, 0.21 M NaCl, 11.5% PEG4000 |
