5EN8
Crystal structure of the N-terminal region of Smu1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.429, 35.233, 79.260 |
Unit cell angles | 90.00, 93.27, 90.00 |
Refinement procedure
Resolution | 40.049 - 2.230 |
R-factor | 0.231 |
Rwork | 0.229 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5en6 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.656 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.049 | 2.360 |
High resolution limit [Å] | 2.220 | 2.220 |
Number of reflections | 21482 | |
<I/σ(I)> | 11.1 | 1.1 |
Completeness [%] | 95.9 | 89.1 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277.15 | 0.1 M MES, pH 6.5, 12 % [w/v] PEG 20,000 |