5EN7
Crystal structure of the Smu1-RED complex (native) of Caenorhabditis elegans.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 122.322, 41.036, 126.382 |
| Unit cell angles | 90.00, 95.92, 90.00 |
Refinement procedure
| Resolution | 46.156 - 2.936 |
| R-factor | 0.2239 |
| Rwork | 0.222 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5en6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.898 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.160 | 3.110 |
| High resolution limit [Å] | 2.936 | 2.940 |
| Number of reflections | 26994 | |
| <I/σ(I)> | 10.6 | 1.4 |
| Completeness [%] | 98.3 | 92.6 |
| Redundancy | 3.3 | 3.5 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291.15 | 0.1 M HEPES, pH 7.5, 0.02 M MgCl2, 22 % [w/v] polyacrilic acid 5,100 |
