5EN6
Crystal structure of the Smu1-RED complex (SeMet) of Caenorhabditis elegans
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979531, 0.979771, 0.977427 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 166.669, 183.443, 41.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.861 - 3.103 |
| R-factor | 0.2849 |
| Rwork | 0.283 |
| R-free | 0.32060 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.722 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.860 | 3.290 |
| High resolution limit [Å] | 3.092 | 3.100 |
| Rmerge | 1.283 | |
| Number of reflections | 11889 | |
| <I/σ(I)> | 19.83 | 2.04 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 12.8 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291.15 | 0.1 M HEPES, pH 7.0, 0.02 M MgCl2, 19 % (w/v) polyacrilic acid 5,100 |
