5EN0
Crystal Structure of T94I rhodopsin mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 242.269, 242.269, 111.147 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.810 |
| R-factor | 0.20937 |
| Rwork | 0.207 |
| R-free | 0.25314 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a4m |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.118 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.980 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.844 |
| Number of reflections | 30386 | |
| <I/σ(I)> | 11 | 1.7 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 5.1 | 5.2 |
| CC(1/2) | 0.996 | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | Ammonium sulfate, Sodium acetate |
