5EMQ

Transcription factor GRDBD and GRE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-10
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.100
Spacegroup name	P 21 21 21
Unit cell lengths	39.032, 100.384, 111.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.361 - 2.300
R-factor	0.2011
Rwork	0.200
R-free	0.22430
RMSD bond length	0.009
RMSD bond angle	1.141
Data scaling software	XSCALE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.796		2.500
High resolution limit [Å]	2.300	10.000	2.300
Rmerge	0.065	0.027	0.453
Rmeas	0.079	0.033	0.550
Total number of observations	56936
Number of reflections	19855	266	4355
<I/σ(I)>	10.99	28.5	2.67
Completeness [%]	97.9	90.2	99.4
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	50mM Na Cacodylate, 2.25mM Spermine, 18mM MgCl2, 9% isopropanol