5EML

Crystal structure of PRMT5:MEP50 with Compound 10 and SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-06-20
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97872
Spacegroup name	I 2 2 2
Unit cell lengths	103.400, 138.130, 178.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.370 - 2.390
R-factor	0.212
Rwork	0.208
R-free	0.28210
RMSD bond length	0.009
RMSD bond angle	1.413
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.1.22)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.060	42.060	2.450
High resolution limit [Å]	2.390	10.690	2.390
Rmerge	0.078	0.030	0.932
Rpim	0.030	0.013	0.361
Total number of observations	380569	4069	27992
Number of reflections	50789
<I/σ(I)>	19.4	58	2.8
Completeness [%]	99.6	97.7	98.9
Redundancy	7.5	6.4	7.6
CC(1/2)	0.999	0.999	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Sodium acetate, 0.1 M Sodium citrate pH 6.1, 10% w/v PEG 4000