5EM9
Crystal structure of the SNX27 PDZ domain bound to the phosphorylated C-terminal 5HT4(a)R PDZ binding motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.219, 48.753, 57.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.066 - 1.600 |
| R-factor | 0.1701 |
| Rwork | 0.169 |
| R-free | 0.19770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z8j |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.306 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.117 | 1.970 |
| Number of reflections | 14298 | |
| <I/σ(I)> | 14.4 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.2 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 293 | 40% PEG 400, 20% PEG8000, 0.1 M acetate (pH 4.5) |
