5ELS

Structure of the KH domain of T-STAR in complex with AAAUAA RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-03
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91730
Spacegroup name	C 2 2 21
Unit cell lengths	93.730, 162.220, 113.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.865 - 2.873
R-factor	0.1897
Rwork	0.188
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.461
Data reduction software	xia2
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.865	46.865	2.950
High resolution limit [Å]	2.873	12.850	2.870
Rmerge		0.062	0.745
Rmeas	0.206
Rpim	0.078	0.028	0.321
Total number of observations	137466	1681	10690
Number of reflections	20014
<I/σ(I)>	8.1	17	2.4
Completeness [%]	99.8	98.7	100
Redundancy	6.9	6.3	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	Lithium sulphate, Tris