5ELR

Structure of the KH-QUA2 domain of T-STAR in complex with AAUAAU RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	42.380, 45.560, 151.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.680 - 2.300
R-factor	0.2294
Rwork	0.227
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.816
Data reduction software	xia2
Data scaling software	Aimless (0.2.12)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.560	45.560	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.081	0.053	0.921
Rpim	0.024	0.019	0.267
Total number of observations	173881	1693	12748
Number of reflections	13783
<I/σ(I)>	21.2	43.5	3.2
Completeness [%]	100.0	99.1	100
Redundancy	12.6	8.6	12.8
CC(1/2)	0.998	0.997	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	Imidazole, PEG 8000