5ELQ
Crystal structure of the SNX27 PDZ domain bound to the C-terminal DGKzeta PDZ binding motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 48.940, 54.120, 74.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.900 - 1.100 |
| R-factor | 0.1636 |
| Rwork | 0.163 |
| R-free | 0.18030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z8j |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.271 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.900 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.099 | 0.820 |
| Number of reflections | 80541 | |
| <I/σ(I)> | 14.5 | 3.3 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 13.2 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 2.0 M Ammonium citrate, 5% PEG400 |
