5ELP
Ketosynthase from module 1 of the bacillaene synthase from Bacillus amyloliquefaciens FZB42
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.620, 99.950, 100.840 |
| Unit cell angles | 91.93, 88.18, 96.04 |
Refinement procedure
| Resolution | 99.350 - 2.930 |
| R-factor | 0.27139 |
| Rwork | 0.270 |
| R-free | 0.30252 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.094 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 99.350 |
| High resolution limit [Å] | 2.930 |
| Number of reflections | 46279 |
| <I/σ(I)> | 15.7 |
| Completeness [%] | 98.7 |
| Redundancy | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 32% PEG 400 (v/v), 0.2 M MgCl2, 100 mM HEPES, pH 7.5 |
