5ELM
Crystal structure of L-aspartate/glutamate specific racemase in complex with L-glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.530, 81.250, 83.960 |
| Unit cell angles | 90.00, 111.39, 90.00 |
Refinement procedure
| Resolution | 29.320 - 2.000 |
| R-factor | 0.17644 |
| Rwork | 0.173 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ell |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.738 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.210 | |
| Number of reflections | 56118 | |
| <I/σ(I)> | 6 | 2.9 |
| Completeness [%] | 98.3 | 94.9 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.3 M ammonium nitrate, 14~16% PEG 3350 |
