5EL7

Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position and antibiotic paromomycin

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	209.700, 449.500, 620.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	152.167 - 3.150
R-factor	0.1917
Rwork	0.192
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wq1
RMSD bond length	0.011
RMSD bond angle	1.842
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	175.000		3.150
High resolution limit [Å]	3.050	100.000	3.050
Rmerge	0.302	0.055	5.075
Rmeas	0.304	0.056	5.131
Total number of observations	82658831
Number of reflections	1099625	24	100723
<I/σ(I)>	13.29	56.31	1
Completeness [%]	100.0	52.2	100
Redundancy	75.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 20000, PEG 550mme, KSCN, Tris-OAc