5EL7
Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position and antibiotic paromomycin
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.700, 449.500, 620.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 152.167 - 3.150 |
| R-factor | 0.1917 |
| Rwork | 0.192 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wq1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.842 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 175.000 | 3.150 | |
| High resolution limit [Å] | 3.050 | 100.000 | 3.050 |
| Rmerge | 0.302 | 0.055 | 5.075 |
| Rmeas | 0.304 | 0.056 | 5.131 |
| Total number of observations | 82658831 | ||
| Number of reflections | 1099625 | 24 | 100723 |
| <I/σ(I)> | 13.29 | 56.31 | 1 |
| Completeness [%] | 100.0 | 52.2 | 100 |
| Redundancy | 75.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 20000, PEG 550mme, KSCN, Tris-OAc |
