5EL5
Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 208.400, 447.100, 616.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 152.436 - 3.150 |
| R-factor | 0.1978 |
| Rwork | 0.198 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wq1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.728 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 200.000 | 3.250 | |
| High resolution limit [Å] | 3.150 | 50.000 | 3.150 |
| Rmerge | 0.231 | 0.048 | 4.799 |
| Rmeas | 0.233 | 0.048 | 4.872 |
| Total number of observations | 55505436 | ||
| Number of reflections | 982379 | 286 | 87342 |
| <I/σ(I)> | 12.88 | 113.76 | 1.07 |
| Completeness [%] | 100.0 | 91.4 | 100 |
| Redundancy | 56.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 20000, PEG 550mme, KSCN, Tris-OAc |
