5EL3

Structure of the KH domain of T-STAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	57.120, 85.920, 59.070
Unit cell angles	90.00, 117.23, 90.00

Refinement procedure

Resolution	52.520 - 1.590
R-factor	0.1779
Rwork	0.176
R-free	0.20500
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.881
Data reduction software	xia2
Data scaling software	Aimless (0.1.27)
Phasing software	AutoSol (1.8.4)
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.520	52.520	1.630
High resolution limit [Å]	1.590	7.120	1.590
Rmerge	0.087	0.062	0.677
Rpim	0.024	0.018	0.190
Total number of observations	924863	10241	67158
Number of reflections	67734
<I/σ(I)>	27.8	102	4
Completeness [%]	99.9	99.9	99.9
Redundancy	13.7	12.8	13.5
CC(1/2)	0.999	0.999	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	Ammonium Sulfate, HEPES, PEG 3350