5EKQ
The structure of the BamACDE subcomplex from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-19 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 234.848, 109.231, 103.988 |
| Unit cell angles | 90.00, 95.04, 90.00 |
Refinement procedure
| Resolution | 29.914 - 3.392 |
| R-factor | 0.2405 |
| Rwork | 0.238 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB IDs 3TGO 4C4V 3Q6B 2KM7 3EFC |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.973 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2142: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.520 |
| High resolution limit [Å] | 3.392 | 7.320 | 3.392 |
| Rmerge | 0.119 | 0.062 | |
| Rmeas | 0.129 | 0.068 | |
| Rpim | 0.058 | 0.028 | 0.875 |
| Total number of observations | 226289 | ||
| Number of reflections | 36099 | ||
| <I/σ(I)> | 5.8 | ||
| Completeness [%] | 99.6 | 98.5 | 99.9 |
| Redundancy | 6.3 | 5.7 | 6.4 |
| CC(1/2) | 0.996 | 0.485 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 100 mM Tris-HCl, pH 8.5, 200 mM MgCl2, 10 mM MnCl2, and 8% PEG 4000 |
