5EKL
Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D/N100E at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2015-07-02 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.927, 59.855, 79.829 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.890 - 2.100 |
R-factor | 0.1883 |
Rwork | 0.185 |
R-free | 0.24420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.865 |
Data reduction software | APEX 2 |
Data scaling software | APEX 2 |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.130 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.042 | 0.172 |
Total number of observations | 138608 | |
Number of reflections | 9178 | |
<I/σ(I)> | 20.71 | 5.58 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.52 | 8.83 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |