5EKI
Crystal Structure of Truncated CCL21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.752, 58.244, 66.054 |
| Unit cell angles | 90.00, 119.94, 90.00 |
Refinement procedure
| Resolution | 57.240 - 1.900 |
| R-factor | 0.21267 |
| Rwork | 0.210 |
| R-free | 0.26219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.836 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.240 | 1.930 |
| High resolution limit [Å] | 1.903 | 1.900 |
| Number of reflections | 34110 | |
| <I/σ(I)> | 16.54 | 2.2 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Ammonium sulfate, MPD |
