5EJZ
Bacterial Cellulose Synthase Product-Bound State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-23 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.269, 216.838, 221.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.351 - 2.940 |
| R-factor | 0.2074 |
| Rwork | 0.206 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p02 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.247 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 3.010 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Rmerge | 0.120 | 0.959 |
| Number of reflections | 68897 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 98.6 | 82.4 |
| Redundancy | 5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 304 | 1.9M NaOAc, Na Citrate |
