5EJU
Ensemble refinement of the Crystal Structure of the Reversibly photoswitching chromoprotein Dathail, Ground State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.970, 81.090, 39.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.270 - 1.650 |
| R-factor | 0.1542 |
| Rwork | 0.151 |
| R-free | 0.19360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.155 |
| Data reduction software | MOSFLM |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.270 | 1.709 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.044 | 0.212 |
| Number of reflections | 29380 | |
| <I/σ(I)> | 18.08 | 4.09 |
| Completeness [%] | 97.7 | 87.25 |
| Redundancy | 5.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Magnesium Chloride, 0.1M Bis-Tris:HCl pH 6.5, 25% PEG 3350 |
