5EJR
Structure of Dictyostelium Discoideum Myosin VII MyTH4-FERM MF2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.020, 58.950, 64.360 |
| Unit cell angles | 90.07, 96.78, 108.48 |
Refinement procedure
| Resolution | 41.220 - 2.000 |
| R-factor | 0.1808 |
| Rwork | 0.179 |
| R-free | 0.21790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in house model |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (2.5.6) |
| Refinement software | BUSTER-TNT (BUSTER 2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 36454 | |
| <I/σ(I)> | 10.9 | 2.14 |
| Completeness [%] | 97.5 | 95.07 |
| Redundancy | 2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 18% PEG 1500, 0.1M MMT (DL-Malic Acid-MES-Tris) pH 8.0 |
