5EJQ
Structure of Dictyostelium Discoideum Myosin VII MyTH4-FERM MF1 domain, mutant 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.320, 61.480, 172.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.010 - 2.190 |
R-factor | 0.1868 |
Rwork | 0.185 |
R-free | 0.21710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ejy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER (2.5.6) |
Refinement software | BUSTER-TNT (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.360 | 2.320 |
High resolution limit [Å] | 2.190 | 2.190 |
Number of reflections | 29999 | |
<I/σ(I)> | 18.15 | 6.14 |
Completeness [%] | 100.0 | 99.97 |
Redundancy | 8.8 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 10% PEG 20K, 20% PEG 550 MME, 0.1M MOPS pH 7.5, 0.1M HEPES pH 7.5, 20 mM DL-Alanine, 20 mM DL-Glutamic acid, 20 mM Glycine, 20 mM DL-Lysine HCl, 20 mM DL-Serine |