5EJD
The crystal structure of holo T3CT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.194, 204.760, 126.047 |
| Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.490 |
| R-factor | 0.19537 |
| Rwork | 0.194 |
| R-free | 0.22164 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dij |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.668 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.580 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.750 | |
| Number of reflections | 182212 | |
| <I/σ(I)> | 5.7 | 1.83 |
| Completeness [%] | 99.2 | 96.8 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | HEPES, magnesium chloride, Sodium polyacrylate 5100 |
