5EJ0
The vaccinia virus H3 envelope protein, a major target of neutralizing antibodies, exhibits a glycosyltransferase fold and binds UDP-Glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-07-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.360, 54.790, 111.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.216 - 1.900 |
| R-factor | 0.2007 |
| Rwork | 0.197 |
| R-free | 0.24000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.089 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.220 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.059 |
| Number of reflections | 21213 |
| <I/σ(I)> | 18.54 |
| Completeness [%] | 99.8 |
| Redundancy | 4.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 100 mM citric acid, 10-20% PEG 6000, 5% 1,3-propanediol and mixture of low molecular mass alcohols |
