5EIY

Bacterial cellulose synthase bound to a substrate analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-15
Detector	ADSC QUANTUM 1
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	68.475, 216.654, 221.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.920 - 2.950
R-factor	0.2229
Rwork	0.222
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p00
RMSD bond length	0.003
RMSD bond angle	0.905
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	35.000
High resolution limit [Å]	2.900
Rmerge	0.280
Number of reflections	133706
<I/σ(I)>	5.2
Completeness [%]	99.3
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	300	sodium acetate