5EIQ

Human OSCAR ligand-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	26.938, 48.486, 74.945
Unit cell angles	90.00, 91.82, 90.00

Refinement procedure

Resolution	40.700 - 2.010
R-factor	0.1808
Rwork	0.179
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p2t
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.486	48.486	2.110
High resolution limit [Å]	2.006	6.340	2.010
Rmerge		0.047	0.650
Rmeas	0.190
Rpim	0.100	0.042	0.489
Total number of observations	47065	1500	6805
Number of reflections	12996
<I/σ(I)>	7.7	18.9	1.9
Completeness [%]	99.1	99.4	98.4
Redundancy	3.6	3.4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	277	0.1 M NaCitrate pH 5.0, 30% PEG 8000