5EIP

apo-structure of YTH domain of SpMmi1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-03
Detector	RIGAKU
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	33.836, 60.261, 119.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.810 - 1.490
R-factor	0.1954
Rwork	0.194
R-free	0.22980
RMSD bond length	0.021
RMSD bond angle	2.003
Data reduction software	Coot
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	59.810
High resolution limit [Å]	1.490
Number of reflections	40097
<I/σ(I)>	16.96
Completeness [%]	98.5
Redundancy	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20% PEG3350, 0.1M Bis-Tris