5EII

Structural determination of an protein complex of a Fab with increased solubility

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	IMAGE PLATE
Collection date	2013-12-13
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	81.486, 62.945, 161.828
Unit cell angles	90.00, 94.00, 90.00

Refinement procedure

Resolution	46.384 - 2.440
R-factor	0.1875
Rwork	0.185
R-free	0.23260
RMSD bond length	0.004
RMSD bond angle	0.716
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.384
High resolution limit [Å]	2.437
Number of reflections	61283
<I/σ(I)>	12.1
Completeness [%]	99.4
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		293	Crystals were grown by hanging drop liquid diffusion by adding a solution containing 20% PEG 3350 and Sodium Acetate, pH 5.5