5EHK
Crystal structure of tRNA dependent lantibiotic dehydratase MibB from Microbispora sp. 107891
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-05-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.137, 151.112, 205.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.239 - 2.708 |
R-factor | 0.2068 |
Rwork | 0.204 |
R-free | 0.26440 |
Structure solution method | MIR |
RMSD bond length | 0.009 |
RMSD bond angle | 1.360 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.065 | 0.772 |
Number of reflections | 72174 | |
<I/σ(I)> | 26.6 | 2 |
Completeness [%] | 99.0 | 99.8 |
Redundancy | 5.9 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100 mM HEPES pH 7.5, 20-24% ethanol; 100 mM MOPS pH 7.9, 20-24% ethanol |