5EHA
Crystal structure of recombinant MtaL at 1.35 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 56.975, 61.337, 59.055 |
Unit cell angles | 90.00, 108.36, 90.00 |
Refinement procedure
Resolution | 47.010 - 1.350 |
R-factor | 0.16324 |
Rwork | 0.162 |
R-free | 0.18315 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y9w |
RMSD bond length | 0.027 |
RMSD bond angle | 2.432 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.010 | 1.420 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.038 | |
Number of reflections | 41973 | |
<I/σ(I)> | 2.7 | |
Completeness [%] | 99.2 | 98.8 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M bis-tris buffer and 2 M ammonium sulfate |