5EHA
Crystal structure of recombinant MtaL at 1.35 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 56.975, 61.337, 59.055 |
| Unit cell angles | 90.00, 108.36, 90.00 |
Refinement procedure
| Resolution | 47.010 - 1.350 |
| R-factor | 0.16324 |
| Rwork | 0.162 |
| R-free | 0.18315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y9w |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.432 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.010 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.038 | |
| Number of reflections | 41973 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M bis-tris buffer and 2 M ammonium sulfate |
