5EGG

Crystal structure of human ubiquitin-conjugating enzyme UBCH5C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97852
Spacegroup name	P 2 21 21
Unit cell lengths	28.170, 66.280, 76.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.420 - 1.760
R-factor	0.2082
Rwork	0.206
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x23
RMSD bond length	0.011
RMSD bond angle	1.316
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	AUTOMAR
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.420	1.860
High resolution limit [Å]	1.760	1.760
Number of reflections	14797
<I/σ(I)>	5.5
Completeness [%]	99.5
Redundancy	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	2% v/v Tacsimate pH 4.0, 0.1 M Sodium acetate trihydrate pH 4.6, 16% w/v Polyethylene glycol 3350