5EFY
Apo-form of SCO3201
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-11 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.790, 98.426, 89.611 |
| Unit cell angles | 90.00, 100.78, 90.00 |
Refinement procedure
| Resolution | 88.030 - 2.700 |
| R-factor | 0.26037 |
| Rwork | 0.258 |
| R-free | 0.29733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cgr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.443 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.600 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.500 | |
| Number of reflections | 23495 | |
| <I/σ(I)> | 7.7 | 1.44 |
| Completeness [%] | 96.6 | |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 12 % Ethanol; 0.1 M Tris/HCl; pH 5 |
