5EFT
Structural Basis for Specific Recognition of ssDNA by SRBSDV P9-1 Octamers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 195 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2015-07-10 |
| Detector | RIGAKU AFC9 |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 118.520, 155.720, 157.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 - 2.500 |
| R-factor | 0.255 |
| Rwork | 0.253 |
| R-free | 0.28420 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.314 |
| Data reduction software | Coot |
| Data scaling software | HKL-2000 |
| Phasing software | Coot |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.600 |
| High resolution limit [Å] | 2.180 |
| Number of reflections | 65431 |
| <I/σ(I)> | 10.1 |
| Completeness [%] | 99.3 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM Trimethylamine N-oxide dihydrate, 100 mM Tris-HCl, 16% Polyethylene glycol monomethyl ether 3000 |
