5EFR
Crystal Structure of a BamA-BamD fusion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.909, 48.062, 78.027 |
| Unit cell angles | 90.00, 104.31, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.2121 |
| Rwork | 0.211 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qky |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.581 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2152) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.420 | 2.000 |
| Rmerge | 0.070 | 0.032 | 0.662 |
| Rmeas | 0.083 | 0.038 | 0.783 |
| Rpim | 0.044 | 0.020 | 0.414 |
| Total number of observations | 116652 | ||
| Number of reflections | 33843 | ||
| <I/σ(I)> | 13.6 | ||
| Completeness [%] | 98.4 | 98.6 | 99.1 |
| Redundancy | 3.4 | 3.6 | 3.5 |
| CC(1/2) | 0.998 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 10% PEG-3000, 15% 2-Propanol and 0.1M HEPES pH 5.6 |
