5EFA

Structure of Influenza B Lee PB2 cap-binding domain bound to m7GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-06-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	40.343, 46.709, 87.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.658 - 1.900
R-factor	0.1786
Rwork	0.176
R-free	0.22830
RMSD bond length	0.008
RMSD bond angle	1.333
Data scaling software	Aimless (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	87.790	87.790	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge	0.120	0.050	0.734
Rpim	0.050	0.022	0.370
Total number of observations	87469	2950	8127
Number of reflections	13441
<I/σ(I)>	11.3	26.9	1.9
Completeness [%]	98.2	99.7	87.9
Redundancy	6.5	5.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	42% PEG400, 0.1 M Hepes pH 7.5