5EFA
Structure of Influenza B Lee PB2 cap-binding domain bound to m7GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-06-25 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.542 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.343, 46.709, 87.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.658 - 1.900 |
R-factor | 0.1786 |
Rwork | 0.176 |
R-free | 0.22830 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.333 |
Data scaling software | Aimless (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 87.790 | 87.790 | 2.000 |
High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
Rmerge | 0.120 | 0.050 | 0.734 |
Rpim | 0.050 | 0.022 | 0.370 |
Total number of observations | 87469 | 2950 | 8127 |
Number of reflections | 13441 | ||
<I/σ(I)> | 11.3 | 26.9 | 1.9 |
Completeness [%] | 98.2 | 99.7 | 87.9 |
Redundancy | 6.5 | 5.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 42% PEG400, 0.1 M Hepes pH 7.5 |