5EF9

Structure of Influenza B Lee PB2 cap-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	40.857, 46.876, 87.677
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.839 - 1.700
R-factor	0.1807
Rwork	0.178
R-free	0.22580
RMSD bond length	0.007
RMSD bond angle	1.098
Data scaling software	Aimless (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	87.680	87.680	1.790
High resolution limit [Å]	1.700	5.380	1.700
Rmerge	0.128	0.043	0.940
Rpim	0.052	0.019	0.377
Total number of observations	134953	4281	19748
Number of reflections	19223
<I/σ(I)>	11.3	27	2.3
Completeness [%]	100.0	100	99.9
Redundancy	7	6.1	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	42% PEG400, 0.1 M Hepes pH 7.5