5EF5
Crystal structure of Chaetomium thermophilum Raptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00003, 0.99188 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 183.830, 183.830, 272.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.984 - 4.300 |
| R-factor | 0.3719 |
| Rwork | 0.371 |
| R-free | 0.39050 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.019 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (dev_1992) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.370 |
| High resolution limit [Å] | 4.300 | 4.300 |
| Rmerge | 0.150 | 1.271 |
| Number of reflections | 32407 | |
| <I/σ(I)> | 9.1 | 1.5 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 303 | 3-dimensional Crystals of 0.15 mm - 0.25 mm in size grew within 5-7 days |
