5EF0

RADIATION DAMAGE TO THE TRAP-RNA COMPLEX: DOSE (DWD) 16.7 MGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.940
Spacegroup name	C 1 2 1
Unit cell lengths	141.160, 111.130, 138.180
Unit cell angles	90.00, 117.39, 90.00

Refinement procedure

Resolution	58.652 - 1.980
R-factor	0.2263
Rwork	0.225
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eeu
RMSD bond length	0.024
RMSD bond angle	2.276
Data reduction software	MOSFLM
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.670	62.670	2.010
High resolution limit [Å]	1.980	10.840	1.980
Rmerge	0.117	0.024	1.841
Rpim	0.071	0.015	1.147
Total number of observations	477213	2890	21958
Number of reflections	130870
<I/σ(I)>	7.4	25.1	0.7
Completeness [%]	99.3	95.6	97.9
Redundancy	3.6	3.6	3.4
CC(1/2)	0.997	0.999	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293.15	Potassium phosphate,L-tryptophan,potassium glutamate,triethanolamine,MgCl2,monomethyl ether PEG 2000